Chromogranin A antibody

Catalog No. ABIN238334

The Mouse Monoclonal anti-Chromogranin A antibody has been validated for IHC and FACS. It is suitable to detect Chromogranin A in samples from Human.

Quick Overview for Chromogranin A antibody (ABIN238334)

Target: Chromogranin A (CHGA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: NJ-5C9

Product Details anti-Chromogranin A Antibody

Purification: Protein G

Immunogen: genetic immunisation with cDNA encoding CGA

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
ELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: short term: 2 °C - 8 °C, long term: -20 °C

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: Chromogranin A

Background: Chromogranin A (CgA) is a 49 kDa protein which is a member of the granin/secretogranin family of acidic glycoproteins. It is specifically expressed in all endocrine and neuroendocrine cells and plays multiple roles in the process of regulated secretion of peptide hormones and neurotransmitters. Owing to its over-expression in several tumors of neuroendocrine origin, CgA is of diagnostic value in classical endocrine, in hormone-negative and in neuroendocrine tumors.

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling